Detailed information of TA system TA029293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 423922..424749
• Accession: NC_020887
• Organism: Clostridium stercorarium subsp. stercorarium DSM 8532

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: CLST_RS01810
• Protein ID: WP_015484857.1
• Coordinates: 423922..424386 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: L7VL72
• Locus tag: CLST_RS01815
• Protein ID: WP_015358106.1
• Coordinates: 424408..424749 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLST_RS01785	419011..419442	+	432	WP_015358101.1	hypothetical protein	-
CLST_RS01790	420326..420457	-	132	Protein_339	helix-turn-helix domain-containing protein	-
CLST_RS13145	420665..420883	+	219	Protein_340	helix-turn-helix domain-containing protein	-
CLST_RS01795	420892..422118	-	1227	WP_015358103.1	IS256 family transposase	-
CLST_RS13275	422179..422331	+	153	Protein_342	IS30 family transposase	-
CLST_RS01805	422700..423917	-	1218	WP_015358104.1	site-specific integrase	-
CLST_RS01810	423922..424386	-	465	WP_015484857.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CLST_RS01815	424408..424749	-	342	WP_015358106.1	helix-turn-helix domain-containing protein	Antitoxin
CLST_RS01820	424896..425096	+	201	WP_015358107.1	transcriptional regulator	-
CLST_RS01825	425136..425330	+	195	WP_015484858.1	helix-turn-helix domain-containing protein	-
CLST_RS01830	425352..425549	+	198	WP_015358108.1	hypothetical protein	-
CLST_RS01835	425705..425884	+	180	WP_015358109.1	hypothetical protein	-
CLST_RS01840	425943..426872	+	930	WP_015358110.1	YqaJ viral recombinase family protein	-
CLST_RS01845	426887..427702	+	816	WP_015358111.1	recombination protein RecT	-
CLST_RS01850	428133..429011	+	879	WP_015358113.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	420734..460638	39904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (14-120)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17967.60 Da
• Isoelectric Point: 6.9505

>T29293 WP_015484857.1 NC_020887:c424386-423922 [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
MLYEFLLIKADNEGINVYEKEIGKLKGLYFDGNIIINSSIETNSEKACILAEELGHHYTTHGNILNQCKVENRKQERRAR
AWAYERLVSLNSLIEASKHCIRNRFELAEFLGVSERFIDEAIQYYKEKYGICHKVGDYIVYFEPLGVLKKVQKE

Nucleotide

• Length: 465 bp

>T29293 NC_020887:c424386-423922 [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
TTGCTTTATGAATTTCTTCTCATCAAGGCCGACAACGAAGGTATAAATGTATATGAAAAGGAAATCGGGAAATTAAAAGG
ATTATACTTTGACGGAAATATAATAATTAATTCTTCAATCGAAACAAATTCTGAAAAAGCCTGTATTTTAGCCGAAGAAT
TGGGACATCATTATACGACCCATGGAAATATATTGAACCAATGTAAAGTAGAAAACAGAAAACAGGAACGTCGGGCCCGC
GCTTGGGCATACGAACGACTGGTAAGTTTGAATAGCCTGATAGAGGCATCTAAACATTGTATCAGAAATAGATTTGAGTT
AGCTGAATTTTTGGGTGTATCCGAACGTTTTATTGATGAGGCAATTCAATATTACAAAGAAAAGTATGGTATTTGCCACA
AAGTTGGAGATTACATAGTTTATTTTGAACCTTTAGGAGTTCTAAAAAAAGTACAAAAGGAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12856.79 Da
• Isoelectric Point: 8.3707

>AT29293 WP_015358106.1 NC_020887:c424749-424408 [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
MAIGDRIKKLRIEKGMTQEELAKYIDSTKQTIYKYENNIVTNIPSDKIEKIAEALGTTPSYLMGWNDTASKNNVKKPITV
AAHIPDGVELTEEDIKQINDFIQFIISKKKDQK

Nucleotide

• Length: 342 bp

>AT29293 NC_020887:c424749-424408 [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
TTGGCTATTGGTGATAGAATAAAAAAACTCAGAATAGAAAAAGGCATGACACAGGAAGAATTGGCCAAATATATTGATTC
CACCAAACAAACAATTTACAAATATGAAAATAACATTGTGACTAATATTCCCTCTGATAAGATTGAAAAAATTGCAGAGG
CATTAGGAACGACACCTTCTTATTTAATGGGCTGGAACGATACAGCTTCCAAAAATAATGTTAAAAAGCCCATCACTGTT
GCCGCACATATTCCTGATGGCGTTGAACTTACTGAGGAAGATATAAAACAAATCAATGACTTCATACAGTTCATTATTTC
AAAAAAGAAGGATCAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: L7VL72