Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 358275..359075 Replicon chromosome
Accession NZ_LS992190
Organism Escherichia coli isolate Escherichia coli str. TO148

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag ECTO148_RS01725 Protein ID WP_000342452.1
Coordinates 358548..359075 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag ECTO148_RS01720 Protein ID WP_001277107.1
Coordinates 358275..358541 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO148_RS01700 353934..354602 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
ECTO148_RS01705 354595..355653 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
ECTO148_RS01710 355898..356752 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
ECTO148_RS01715 357023..358126 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
ECTO148_RS01720 358275..358541 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
ECTO148_RS01725 358548..359075 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
ECTO148_RS01730 359072..359455 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
ECTO148_RS01735 359878..360987 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
ECTO148_RS01740 361035..361961 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ECTO148_RS01745 361958..363235 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
ECTO148_RS01750 363232..363999 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T292911 WP_000342452.1 NZ_LS992190:358548-359075 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT292911 WP_001277107.1 NZ_LS992190:358275-358541 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References