Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1747627..1748149 Replicon chromosome
Accession NC_020834
Organism Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag D648_RS08710 Protein ID WP_006249269.1
Coordinates 1747853..1748149 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag D648_RS08705 Protein ID WP_006249270.1
Coordinates 1747627..1747869 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D648_RS08680 1743465..1744554 - 1090 Protein_1662 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
D648_RS08685 1744728..1745369 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
D648_RS08690 1745492..1746334 - 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
D648_RS08695 1746394..1746969 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
D648_RS08700 1746947..1747615 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
D648_RS08705 1747627..1747869 - 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
D648_RS08710 1747853..1748149 - 297 WP_006249269.1 BrnT family toxin Toxin
D648_RS08715 1748276..1751188 - 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
D648_RS08720 1751349..1751648 - 300 WP_006249265.1 hypothetical protein -
D648_RS08725 1751769..1753094 - 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T29291 WP_006249269.1 NC_020834:c1748149-1747853 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T29291 NC_020834:c1748149-1747853 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT29291 WP_006249270.1 NC_020834:c1747869-1747627 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT29291 NC_020834:c1747869-1747627 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References