Detailed information of TA system TA029290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1037813..1038453
• Replicon: chromosome
• Accession: NC_020834
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: D648_RS05220
• Protein ID: WP_006250075.1
• Coordinates: 1038271..1038453 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: D648_RS05215
• Protein ID: WP_006250074.1
• Coordinates: 1037813..1038232 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D648_RS05190	1033204..1034010	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
D648_RS05195	1034060..1035466	+	1407	WP_006253513.1	YdgA family protein	-
D648_RS05200	1035533..1036539	-	1007	Protein_973	IS481 family transposase	-
D648_RS05210	1036833..1037477	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
D648_RS05215	1037813..1038232	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D648_RS05220	1038271..1038453	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
D648_RS05225	1038585..1039070	-	486	WP_015484654.1	hypothetical protein	-
D648_RS05230	1039075..1039455	-	381	WP_006248823.1	hypothetical protein	-
D648_RS05235	1039530..1040213	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
D648_RS05240	1040344..1040544	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
D648_RS12885	1040565..1041632	+	1068	WP_006253284.1	hypothetical protein	-
D648_RS12780	1041642..1041800	+	159	WP_006247809.1	hypothetical protein	-
D648_RS05255	1041809..1042108	-	300	WP_006247810.1	XRE family transcriptional regulator	-
D648_RS05260	1042080..1042469	-	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D648_RS05265	1042613..1042906	+	294	WP_006247812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T29290 WP_006250075.1 NC_020834:c1038453-1038271 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T29290 NC_020834:c1038453-1038271 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT29290 WP_006250074.1 NC_020834:c1038232-1037813 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT29290 NC_020834:c1038232-1037813 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9