Detailed information of TA system TA029289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 793805..794401
• Replicon: chromosome
• Accession: NC_020834
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D648_RS04035
• Protein ID: WP_006249776.1
• Coordinates: 794177..794401 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A547EKP0
• Locus tag: D648_RS04030
• Protein ID: WP_006249777.1
• Coordinates: 793805..794134 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D648_RS04005	789254..789943	+	690	WP_006253589.1	GTP cyclohydrolase I FolE	-
D648_RS04010	790008..790793	+	786	WP_006249781.1	tRNA pseudouridine(38-40) synthase TruA	-
D648_RS04015	790846..793038	-	2193	WP_006249780.1	copper-translocating P-type ATPase	-
D648_RS04020	793091..793291	-	201	WP_006249779.1	heavy-metal-associated domain-containing protein	-
D648_RS04025	793380..793766	+	387	WP_006249778.1	Cu(I)-responsive transcriptional regulator	-
D648_RS04030	793805..794134	-	330	WP_006249777.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D648_RS04035	794177..794401	-	225	WP_006249776.1	type II toxin-antitoxin system HicA family toxin	Toxin
D648_RS04040	794609..796516	+	1908	WP_006249774.1	DNA topoisomerase IV subunit B	-
D648_RS04045	796590..797195	-	606	WP_006249773.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
D648_RS04050	797265..797621	+	357	WP_006249772.1	dihydroneopterin aldolase	-
D648_RS04055	797618..798520	+	903	WP_006249771.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-73)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 10.5597

>T29289 WP_006249776.1 NC_020834:c794401-794177 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Nucleotide

• Length: 225 bp

>T29289 NC_020834:c794401-794177 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.06 Da
• Isoelectric Point: 4.7022

>AT29289 WP_006249777.1 NC_020834:c794134-793805 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Nucleotide

• Length: 330 bp

>AT29289 NC_020834:c794134-793805 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A547EKP0