Detailed information of TA system TA292875

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1136764..1137458
• Replicon: chromosome
• Accession: NZ_LS992185
• Organism: Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: EC3426_RS05585
• Protein ID: WP_001094398.1
• Coordinates: 1137090..1137458 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EC3426_RS05580
• Protein ID: WP_077249034.1
• Coordinates: 1136764..1137069 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC3426_RS05535	1132714..1133169	+	456	WP_000581506.1	hypothetical protein	-
EC3426_RS05540	1133270..1133478	+	209	Protein_1042	DUF905 family protein	-
EC3426_RS05550	1133579..1134400	+	822	WP_001234565.1	DUF945 domain-containing protein	-
EC3426_RS05560	1134742..1135215	+	474	WP_133963778.1	antirestriction protein	-
EC3426_RS05565	1135231..1135707	+	477	WP_001424026.1	RadC family protein	-
EC3426_RS05570	1135776..1135997	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EC3426_RS05575	1136016..1136660	+	645	WP_000086755.1	hypothetical protein	-
EC3426_RS05580	1136764..1137069	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EC3426_RS05585	1137090..1137458	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
EC3426_RS05590	1137779..1138117	-	339	Protein_1050	LysR family transcriptional regulator	-
EC3426_RS05595	1138168..1139124	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
EC3426_RS05600	1139134..1141332	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
EC3426_RS05605	1141329..1142285	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1121105..1144003	22898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T292875 WP_001094398.1 NZ_LS992185:1137090-1137458 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT292875 WP_077249034.1 NZ_LS992185:1136764-1137069 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4