Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 237339..237928 Replicon chromosome
Accession NC_020834
Organism Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag D648_RS01240 Protein ID WP_006248516.1
Coordinates 237339..237671 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag D648_RS01245 Protein ID WP_006248517.1
Coordinates 237668..237928 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D648_RS01200 232534..233154 - 621 WP_006248508.1 dTMP kinase -
D648_RS01205 233165..234199 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
D648_RS01225 234779..235306 + 528 WP_006248512.1 inorganic diphosphatase -
D648_RS01230 235362..236117 + 756 WP_006248513.1 M48 family metallopeptidase -
D648_RS01235 236181..237212 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
D648_RS01240 237339..237671 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D648_RS01245 237668..237928 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D648_RS01250 237998..238666 - 669 WP_006248518.1 phosphoglycolate phosphatase -
D648_RS01255 238753..239550 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
D648_RS01260 239558..240346 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
D648_RS01265 240348..240992 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
D648_RS01270 241604..242749 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T29286 WP_006248516.1 NC_020834:c237671-237339 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T29286 NC_020834:c237671-237339 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT29286 WP_006248517.1 NC_020834:c237928-237668 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT29286 NC_020834:c237928-237668 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References