Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2948890..2949480 Replicon chromosome
Accession NZ_LS992183
Organism Citrobacter freundii isolate Citrobacter freundii str. U2785

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag CFU2785_RS14765 Protein ID WP_003836692.1
Coordinates 2948890..2949222 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag CFU2785_RS14770 Protein ID WP_003836694.1
Coordinates 2949223..2949480 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFU2785_RS14740 2944643..2946130 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
CFU2785_RS14750 2946446..2947396 - 951 WP_134215814.1 HTH-type transcriptional regulator Cbl -
CFU2785_RS14755 2947498..2948415 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
CFU2785_RS14765 2948890..2949222 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CFU2785_RS14770 2949223..2949480 - 258 WP_003836694.1 hypothetical protein Antitoxin
CFU2785_RS14780 2950094..2952626 + 2533 Protein_2821 hypothetical protein -
CFU2785_RS14785 2952651..2953682 - 1032 WP_001395480.1 IS630-like element ISEc33 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T292856 WP_003836692.1 NZ_LS992183:c2949222-2948890 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT292856 WP_003836694.1 NZ_LS992183:c2949480-2949223 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References