Detailed information of TA system TA029285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 10412..11127
• Replicon: chromosome
• Accession: NC_020834
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D648_RS00040
• Protein ID: WP_006250491.1
• Coordinates: 10412..10840 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A378MVR3
• Locus tag: D648_RS00045
• Protein ID: WP_015484790.1
• Coordinates: 10840..11127 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D648_RS00025	5668..7320	+	1653	WP_015484793.1	phospho-sugar mutase	-
D648_RS00030	7373..8305	+	933	WP_015484792.1	nucleoside hydrolase	-
D648_RS00035	8327..10408	-	2082	WP_015484791.1	ATP-dependent DNA helicase RecG	-
D648_RS00040	10412..10840	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
D648_RS00045	10840..11127	-	288	WP_015484790.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D648_RS00050	11291..13174	+	1884	WP_015484789.1	molecular chaperone HtpG	-
D648_RS00055	13227..13409	+	183	WP_006250493.1	hypothetical protein	-
D648_RS00060	13573..15288	+	1716	WP_006248268.1	proline--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T29285 WP_006250491.1 NC_020834:c10840-10412 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T29285 NC_020834:c10840-10412 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11097.45 Da
• Isoelectric Point: 4.3648

>AT29285 WP_015484790.1 NC_020834:c11127-10840 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MAIIITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT29285 NC_020834:c11127-10840 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGGCAATAATCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A378MVR3