292825

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4326818..4327394 Replicon chromosome
Accession NZ_LS992180
Organism Escherichia coli isolate Escherichia coli str. TO124

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag ECTO124_RS21670 Protein ID WP_001295743.1
Coordinates 4327107..4327394 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag ECTO124_RS21665 Protein ID WP_000063148.1
Coordinates 4326818..4327120 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO124_RS21650 4323414..4325564 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
ECTO124_RS21655 4325614..4325817 + 204 WP_000467859.1 YbdD/YjiX family protein -
ECTO124_RS21660 4325828..4326784 + 957 WP_001295745.1 GTPase -
ECTO124_RS21665 4326818..4327120 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
ECTO124_RS21670 4327107..4327394 - 288 WP_001295743.1 BrnT family toxin Toxin
ECTO124_RS21675 4327591..4328757 + 1167 WP_000800831.1 restriction endonuclease -
ECTO124_RS21680 4328821..4330440 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
ECTO124_RS21685 4330430..4331734 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T292825 WP_001295743.1 NZ_LS992180:c4327394-4327107 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT292825 WP_000063148.1 NZ_LS992180:c4327120-4326818 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References