Detailed information of TA system TA029282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1417609..1418257
• Replicon: chromosome
• Accession: NC_020833
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-183

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: D650_RS07160
• Protein ID: WP_006251221.1
• Coordinates: 1417609..1417785 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: D650_RS07165
• Protein ID: WP_006251222.1
• Coordinates: 1417841..1418257 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D650_RS07135	1412927..1415422	-	2496	WP_006251216.1	phage tail length tape measure family protein	-
D650_RS07145	1415967..1416425	-	459	WP_006251218.1	hypothetical protein	-
D650_RS07150	1416594..1416827	+	234	WP_020824316.1	hypothetical protein	-
D650_RS07155	1416842..1417513	-	672	WP_006251220.1	hypothetical protein	-
D650_RS07160	1417609..1417785	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
D650_RS07165	1417841..1418257	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D650_RS07170	1418297..1418779	-	483	WP_006251223.1	phage tail protein	-
D650_RS07175	1418783..1419163	-	381	WP_006251224.1	hypothetical protein	-
D650_RS07180	1419160..1419528	-	369	WP_006251225.1	hypothetical protein	-
D650_RS07185	1419530..1419874	-	345	WP_006251226.1	hypothetical protein	-
D650_RS07190	1419874..1420251	-	378	WP_006251227.1	hypothetical protein	-
D650_RS07195	1420254..1420505	-	252	WP_015484272.1	hypothetical protein	-
D650_RS07200	1420517..1421506	-	990	WP_006251229.1	encapsulin	-
D650_RS07205	1421521..1421955	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1393821..1475807	81986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T29282 WP_006251221.1 NC_020833:1417609-1417785 [Mannheimia haemolytica USDA-ARS-USMARC-183]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T29282 NC_020833:1417609-1417785 [Mannheimia haemolytica USDA-ARS-USMARC-183]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT29282 WP_006251222.1 NC_020833:1417841-1418257 [Mannheimia haemolytica USDA-ARS-USMARC-183]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT29282 NC_020833:1417841-1418257 [Mannheimia haemolytica USDA-ARS-USMARC-183]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6