Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1103078..1103718 Replicon chromosome
Accession NC_020833
Organism Mannheimia haemolytica USDA-ARS-USMARC-183

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag D650_RS05650 Protein ID WP_006250075.1
Coordinates 1103536..1103718 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag D650_RS05645 Protein ID WP_006250074.1
Coordinates 1103078..1103497 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D650_RS05625 1098625..1099431 + 807 WP_006248821.1 tryptophan synthase subunit alpha -
D650_RS05630 1099481..1100887 + 1407 WP_006253513.1 YdgA family protein -
D650_RS05635 1100954..1101960 - 1007 Protein_1065 IS481 family transposase -
D650_RS05640 1102098..1102742 - 645 WP_006253274.1 tyrosine-type recombinase/integrase -
D650_RS05645 1103078..1103497 - 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
D650_RS05650 1103536..1103718 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
D650_RS05655 1103850..1104335 - 486 WP_015484250.1 hypothetical protein -
D650_RS05660 1104340..1104720 - 381 WP_006248823.1 hypothetical protein -
D650_RS05665 1104795..1105478 - 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
D650_RS05670 1105609..1105809 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
D650_RS13970 1105830..1106897 + 1068 WP_006253284.1 hypothetical protein -
D650_RS13805 1106907..1107065 + 159 WP_006247809.1 hypothetical protein -
D650_RS05685 1107074..1107373 - 300 WP_006247810.1 XRE family transcriptional regulator -
D650_RS05690 1107345..1107734 - 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
D650_RS05695 1107878..1108171 + 294 WP_006247812.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T29281 WP_006250075.1 NC_020833:c1103718-1103536 [Mannheimia haemolytica USDA-ARS-USMARC-183]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T29281 NC_020833:c1103718-1103536 [Mannheimia haemolytica USDA-ARS-USMARC-183]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT29281 WP_006250074.1 NC_020833:c1103497-1103078 [Mannheimia haemolytica USDA-ARS-USMARC-183]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT29281 NC_020833:c1103497-1103078 [Mannheimia haemolytica USDA-ARS-USMARC-183]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References