Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 32057..32860 Replicon plasmid 2
Accession NZ_LS992176
Organism Citrobacter freundii isolate Citrobacter freundii str. E2614

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag CFE2614_RS25860 Protein ID WP_022652313.1
Coordinates 32330..32860 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag CFE2614_RS25855 Protein ID WP_022652312.1
Coordinates 32057..32326 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFE2614_RS25835 28362..28669 - 308 Protein_27 IS1 family transposase -
CFE2614_RS25845 29146..29886 + 741 WP_022652310.1 site-specific integrase -
CFE2614_RS25850 30212..31201 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
CFE2614_RS25855 32057..32326 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
CFE2614_RS25860 32330..32860 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
CFE2614_RS25865 32992..33885 + 894 WP_077873527.1 IS5 family transposase -
CFE2614_RS25870 34026..34922 - 897 Protein_33 IS3 family transposase -
CFE2614_RS25875 34987..36138 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
CFE2614_RS25880 36247..36630 + 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
CFE2614_RS25885 36627..36974 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaVIM-1 / ant(3'')-Ia / qacE / sul1 / mph(A) / aac(3)-IId / blaTEM-1B - 1..159493 159493
- inside IScluster/Tn - - 27048..40429 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T292806 WP_022652313.1 NZ_LS992176:32330-32860 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT292806 WP_022652312.1 NZ_LS992176:32057-32326 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References