Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4981965..4982581 Replicon chromosome
Accession NZ_LS992175
Organism Citrobacter freundii isolate Citrobacter freundii str. E2614

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag CFE2614_RS24775 Protein ID WP_003028682.1
Coordinates 4981965..4982339 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag CFE2614_RS24780 Protein ID WP_043018956.1
Coordinates 4982339..4982581 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFE2614_RS24760 4979468..4980370 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
CFE2614_RS24765 4980367..4981002 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
CFE2614_RS24770 4980999..4981928 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
CFE2614_RS24775 4981965..4982339 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
CFE2614_RS24780 4982339..4982581 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
CFE2614_RS24785 4982787..4983695 + 909 WP_044701816.1 alpha/beta hydrolase -
CFE2614_RS24795 4983846..4984787 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
CFE2614_RS24800 4984832..4985269 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
CFE2614_RS24805 4985266..4986138 - 873 WP_003028669.1 virulence factor BrkB family protein -
CFE2614_RS24810 4986132..4986731 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T292805 WP_003028682.1 NZ_LS992175:c4982339-4981965 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT292805 WP_043018956.1 NZ_LS992175:c4982581-4982339 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References