Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2243613..2244139 Replicon chromosome
Accession NZ_LS992171
Organism Escherichia coli isolate Escherichia coli str. TO73

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECTO73_RS10965 Protein ID WP_000323025.1
Coordinates 2243852..2244139 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECTO73_RS10960 Protein ID WP_000534858.1
Coordinates 2243613..2243852 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO73_RS10925 2238972..2239319 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
ECTO73_RS10930 2239316..2239720 - 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
ECTO73_RS24255 2239942..2240229 + 288 Protein_2116 hypothetical protein -
ECTO73_RS10945 2240998..2242311 + 1314 WP_000589005.1 ISNCY family transposase -
ECTO73_RS10950 2242748..2243080 - 333 WP_001301033.1 protein FlxA -
ECTO73_RS10955 2243283..2243588 - 306 WP_001326990.1 hypothetical protein -
ECTO73_RS10960 2243613..2243852 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECTO73_RS10965 2243852..2244139 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECTO73_RS10970 2244211..2244366 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECTO73_RS10975 2244583..2244834 + 252 WP_000980994.1 hypothetical protein -
ECTO73_RS10980 2244901..2245179 + 279 WP_012304870.1 hypothetical protein -
ECTO73_RS10985 2245181..2246230 + 1050 WP_001265199.1 DUF968 domain-containing protein -
ECTO73_RS10990 2246244..2246996 + 753 WP_001047135.1 antitermination protein -
ECTO73_RS10995 2247274..2247363 - 90 WP_120795389.1 hypothetical protein -
ECTO73_RS11000 2247418..2247630 - 213 WP_000087756.1 cold shock-like protein CspF -
ECTO73_RS11005 2247931..2248146 + 216 WP_000066484.1 cold shock-like protein CspB -
ECTO73_RS24390 2248510..2248680 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
ECTO73_RS11010 2248900..2249109 + 210 WP_032347816.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2228033..2290957 62924
- inside Prophage - - 2211149..2307154 96005


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T292772 WP_000323025.1 NZ_LS992171:2243852-2244139 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT292772 WP_000534858.1 NZ_LS992171:2243613-2243852 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References