Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 109478..110003 Replicon plasmid 2
Accession NZ_LS992169
Organism Escherichia coli isolate Escherichia coli str. TO60

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ECTO60_RS25740 Protein ID WP_001159868.1
Coordinates 109478..109783 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ECTO60_RS25745 Protein ID WP_000813634.1
Coordinates 109785..110003 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO60_RS25725 105388..106554 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
ECTO60_RS25730 107142..107897 - 756 WP_000852146.1 replication initiation protein RepE -
ECTO60_RS25735 108671..109477 - 807 WP_000016982.1 site-specific integrase -
ECTO60_RS25740 109478..109783 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ECTO60_RS25745 109785..110003 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECTO60_RS25750 110711..111706 + 996 WP_000246636.1 hypothetical protein -
ECTO60_RS25755 111710..112642 + 933 WP_000991832.1 hypothetical protein -
ECTO60_RS25760 112779..113476 - 698 Protein_133 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB / senB 1..172923 172923
- flank IS/Tn - - 112779..113282 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T292758 WP_001159868.1 NZ_LS992169:c109783-109478 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT292758 WP_000813634.1 NZ_LS992169:c110003-109785 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References