Detailed information of TA system TA029274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 509760..510396
• Replicon: chromosome
• Accession: NC_020832
• Organism: Bacillus subtilis subsp. subtilis str. BAB-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: I653_RS02510
• Protein ID: WP_003156187.1
• Coordinates: 510046..510396 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: I653_RS02505
• Protein ID: WP_003225183.1
• Coordinates: 509760..510041 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I653_RS02485	506119..506718	-	600	WP_015482831.1	rhomboid family intramembrane serine protease	-
I653_RS02490	506813..507178	+	366	WP_015252768.1	holo-ACP synthase	-
I653_RS02495	507344..508360	+	1017	WP_080030712.1	outer membrane lipoprotein carrier protein LolA	-
I653_RS02500	508475..509644	+	1170	WP_015382833.1	alanine racemase	-
I653_RS02505	509760..510041	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
I653_RS02510	510046..510396	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I653_RS02515	510512..511336	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
I653_RS02520	511341..511706	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
I653_RS02525	511710..512111	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
I653_RS02530	512123..513130	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
I653_RS02535	513192..513521	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
I653_RS02540	513518..514000	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
I653_RS02545	513966..514754	+	789	WP_015482833.1	RNA polymerase sigma factor SigB	-
I653_RS02550	514754..515353	+	600	WP_015382836.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T29274 WP_003156187.1 NC_020832:510046-510396 [Bacillus subtilis subsp. subtilis str. BAB-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T29274 NC_020832:510046-510396 [Bacillus subtilis subsp. subtilis str. BAB-1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT29274 WP_003225183.1 NC_020832:509760-510041 [Bacillus subtilis subsp. subtilis str. BAB-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT29274 NC_020832:509760-510041 [Bacillus subtilis subsp. subtilis str. BAB-1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32