Detailed information of TA system TA029273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5624461..5625049
• Replicon: chromosome
• Accession: NC_020829
• Organism: Pseudomonas denitrificans ATCC 13867

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: H681_RS26175
• Protein ID: WP_015479720.1
• Coordinates: 5624461..5624763 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: M4X756
• Locus tag: H681_RS25155
• Protein ID: WP_015479721.1
• Coordinates: 5624756..5625049 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H681_RS26170	5619891..5620544	-	654	WP_080636259.1	hypothetical protein	-
H681_RS25135	5620826..5622202	+	1377	WP_015479717.1	L-serine ammonia-lyase	-
H681_RS25140	5622577..5623683	+	1107	WP_015479718.1	GlxA family transcriptional regulator	-
H681_RS25145	5623840..5624244	-	405	WP_015479719.1	VOC family protein	-
H681_RS26175	5624461..5624763	+	303	WP_015479720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H681_RS25155	5624756..5625049	+	294	WP_015479721.1	putative addiction module antidote protein	Antitoxin
H681_RS25160	5625080..5625799	-	720	Protein_5008	DUF637 domain-containing protein	-
H681_RS25165	5625976..5626983	-	1008	WP_015479723.1	IS5 family transposase	-
H681_RS26530	5627113..5627271	-	159	WP_157883359.1	hypothetical protein	-
H681_RS26535	5627528..5627704	+	177	WP_157883360.1	hypothetical protein	-
H681_RS26180	5627762..5628316	-	555	WP_080636260.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (7-93)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11244.93 Da
• Isoelectric Point: 10.6099

>T29273 WP_015479720.1 NC_020829:5624461-5624763 [Pseudomonas sp. ATCC 13867]
MKKIESAKFRTWLQNLKDNRGRARIVARINRLLEGLPGDVAPVGQGVSELRIHHEPGYRVYFHQQGSTLLILLCGGDKGS
QGRDIEAAQKMLAAWRAQHD

Nucleotide

• Length: 303 bp

>T29273 NC_020829:5624461-5624763 [Pseudomonas sp. ATCC 13867]
ATGAAGAAAATCGAGTCCGCCAAGTTTCGAACCTGGCTGCAGAACCTGAAGGACAATAGGGGCAGGGCCCGCATCGTCGC
CCGGATAAACCGCCTGCTGGAAGGCCTGCCAGGCGACGTCGCACCGGTCGGCCAGGGTGTCAGCGAGCTTCGGATACACC
ATGAACCAGGCTACCGCGTCTATTTCCATCAGCAGGGCAGCACTCTGCTGATACTCCTCTGCGGAGGCGACAAGGGCAGC
CAGGGTCGTGACATCGAAGCTGCGCAGAAAATGCTGGCGGCGTGGAGAGCACAACATGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10683.17 Da
• Isoelectric Point: 4.5938

>AT29273 WP_015479721.1 NC_020829:5624756-5625049 [Pseudomonas sp. ATCC 13867]
MTETFTEFDPAQYLTNPEAIAEFMSDALETGDASYIAKAMGVVARAKGMTELARETGLSREQLYRSFSEHGNPTLKTLLA
VMNALGIEMTVRPQLVH

Nucleotide

• Length: 294 bp

>AT29273 NC_020829:5624756-5625049 [Pseudomonas sp. ATCC 13867]
ATGACTGAGACGTTCACCGAATTCGATCCCGCGCAGTACCTCACCAACCCCGAAGCCATTGCCGAGTTCATGAGTGACGC
GCTGGAAACCGGCGATGCCTCCTACATTGCCAAAGCCATGGGAGTCGTCGCTCGCGCCAAAGGCATGACGGAACTGGCGC
GGGAAACCGGCCTGTCTCGCGAGCAGCTCTATCGTTCCTTCAGCGAACACGGCAACCCGACGCTGAAGACGCTACTGGCC
GTGATGAATGCTCTCGGAATCGAAATGACAGTACGTCCCCAACTGGTGCACTAA

Structures

Antitoxin

• AlphaFold DB: M4X756