Detailed information of TA system TA029270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 3965540..3967027
• Replicon: chromosome
• Accession: NC_020829
• Organism: Pseudomonas denitrificans ATCC 13867

Toxin (Protein)

• Gene name: -
• Uniprot ID: M4XJE3
• Locus tag: H681_RS17650
• Protein ID: WP_015478238.1
• Coordinates: 3966458..3967027 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M4X004
• Locus tag: H681_RS17645
• Protein ID: WP_015478237.1
• Coordinates: 3965540..3966391 (-)
• Length: 284 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H681_RS17630	3960838..3961047	+	210	WP_015478234.1	cold-shock protein	-
H681_RS17635	3961114..3962988	-	1875	WP_015478235.1	MFS transporter	-
H681_RS17640	3963169..3965334	-	2166	WP_015478236.1	TonB-dependent receptor family protein	-
H681_RS17645	3965540..3966391	-	852	WP_015478237.1	AraC family transcriptional regulator	Antitoxin
H681_RS17650	3966458..3967027	-	570	WP_015478238.1	GNAT family N-acetyltransferase	Toxin
H681_RS17655	3967293..3970769	+	3477	WP_015478239.1	hybrid sensor histidine kinase/response regulator	-
H681_RS17660	3970928..3971386	-	459	WP_015478240.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (20-140)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21245.49 Da
• Isoelectric Point: 9.5192

>T29270 WP_015478238.1 NC_020829:c3967027-3966458 [Pseudomonas sp. ATCC 13867]
MFRPLPITLQRGALRLEPMVEADVPELVTLAEANREALQYMSAPQRPDWYRQGLADQREGRALPLVVRLGNEIVGTTRFM
DFLPALPACEIGATWLDQAQHGSGLNATMKYLMLRHAFDSWKMVRVQLKTAASNLRSQRAIEKLGAVREGVLRNHRRLAG
GRLDDTILYSITDHEWPQVKAALEAGFNG

Nucleotide

• Length: 570 bp

>T29270 NC_020829:c3967027-3966458 [Pseudomonas sp. ATCC 13867]
ATGTTCAGACCGTTACCGATCACCTTGCAGCGCGGGGCCTTGCGCCTGGAGCCGATGGTGGAGGCCGACGTGCCCGAACT
GGTGACGCTGGCGGAAGCGAACCGCGAGGCGTTGCAGTACATGAGCGCCCCGCAGCGCCCGGACTGGTACCGCCAGGGCC
TGGCCGACCAGCGCGAAGGCCGCGCGCTGCCGCTGGTGGTGCGCCTGGGCAACGAGATCGTCGGCACCACGCGTTTCATG
GACTTTCTCCCGGCGCTGCCGGCCTGCGAGATCGGCGCCACCTGGCTGGACCAGGCGCAACACGGCAGCGGCCTGAACGC
CACCATGAAGTACCTGATGCTGCGCCACGCCTTCGACAGCTGGAAGATGGTCCGCGTGCAACTCAAGACCGCCGCCAGCA
ACCTGCGTTCGCAGCGCGCCATCGAGAAGCTCGGCGCGGTGCGCGAAGGCGTGTTGCGCAACCACCGGCGCCTGGCCGGC
GGTCGGCTCGACGACACTATCCTCTACAGCATCACCGACCACGAGTGGCCGCAGGTGAAGGCCGCGCTGGAAGCCGGTTT
CAACGGCTGA

Antitoxin

Protein

• Length: 284 a.a.
• Molecular weight: 31509.87 Da
• Isoelectric Point: 7.1676

>AT29270 WP_015478237.1 NC_020829:c3966391-3965540 [Pseudomonas sp. ATCC 13867]
MTEHHASGERTRFWRAQELGGVELLHARYIEQVFSPHVHEGFALVMIEQGAQRFHHRGAEHFAPAGSVVLINPDEVHTGS
KADEAGWRYRGFYPELEQVSGVLEELELCGGGTPSFDASVVFDPELVQALFAAHRLVDSDATVLQRQTCWREALLLLFQR
HARIPEAPPAGNEPVAVARARELLGARLMEPPSLEELAAAVGLSPFHFARVFRKATGLPPHAWLRQRRLEQARALLKQGC
MPVSVAMQLGFADQSHLSRQFKQAYGVGPGEYRQACARSFRPV

Nucleotide

• Length: 852 bp

>AT29270 NC_020829:c3966391-3965540 [Pseudomonas sp. ATCC 13867]
ATGACGGAACACCACGCGAGCGGCGAAAGAACCCGCTTCTGGCGAGCCCAGGAGCTGGGGGGCGTCGAGCTGCTGCACGC
GCGCTACATCGAGCAGGTGTTTTCGCCCCACGTCCACGAGGGGTTTGCCCTCGTCATGATCGAGCAGGGCGCGCAGCGCT
TCCACCATCGCGGCGCCGAGCATTTCGCCCCGGCCGGCAGTGTGGTGCTGATCAATCCCGACGAGGTTCACACCGGCTCC
AAGGCCGACGAGGCCGGCTGGCGTTATCGCGGCTTCTATCCGGAGCTGGAGCAGGTCTCCGGCGTACTGGAGGAACTGGA
GCTCTGCGGCGGCGGAACGCCGTCGTTCGATGCCAGCGTGGTGTTCGACCCGGAGCTGGTGCAGGCGCTGTTCGCTGCCC
ACCGCCTGGTGGATTCCGACGCCACCGTGCTGCAACGCCAGACCTGCTGGCGCGAAGCGCTCCTGCTGCTGTTCCAGCGC
CATGCGCGGATTCCCGAAGCGCCGCCGGCCGGCAACGAGCCGGTGGCCGTGGCGCGGGCGCGGGAACTGCTCGGCGCGCG
GCTGATGGAGCCGCCGTCGCTGGAAGAGCTGGCCGCTGCCGTCGGGCTGTCGCCTTTCCATTTCGCCCGCGTGTTCCGCA
AGGCCACCGGCCTGCCGCCCCATGCCTGGCTCAGGCAACGCCGGCTGGAGCAGGCAAGGGCGCTGCTCAAGCAGGGCTGC
ATGCCGGTGAGTGTGGCGATGCAGCTGGGTTTCGCCGACCAGAGTCACCTCAGCCGGCAGTTCAAGCAGGCCTATGGCGT
CGGCCCCGGCGAATACCGCCAGGCTTGTGCGCGGTCTTTCCGTCCCGTCTGA

Structures

Toxin

• AlphaFold DB: M4XJE3

Antitoxin

• AlphaFold DB: M4X004