Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 762611..763200 Replicon chromosome
Accession NC_020829
Organism Pseudomonas denitrificans ATCC 13867

Toxin (Protein)


Gene name hicA Uniprot ID M4WV53
Locus tag H681_RS03555 Protein ID WP_015475462.1
Coordinates 762611..762862 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID M4WRA3
Locus tag H681_RS03560 Protein ID WP_015475463.1
Coordinates 762859..763200 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H681_RS03535 757672..758649 + 978 WP_015475458.1 nitronate monooxygenase family protein -
H681_RS03540 758714..759964 + 1251 WP_015475459.1 OprD family porin -
H681_RS03545 760099..760782 + 684 WP_015475460.1 TetR/AcrR family transcriptional regulator -
H681_RS03550 760779..762428 + 1650 WP_015475461.1 acyl-CoA dehydrogenase family protein -
H681_RS03555 762611..762862 + 252 WP_015475462.1 type II toxin-antitoxin system HicA family toxin Toxin
H681_RS03560 762859..763200 + 342 WP_015475463.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H681_RS03565 763455..764930 + 1476 WP_015475464.1 AMP nucleosidase -
H681_RS03570 765013..765789 + 777 WP_015475465.1 deoxyribose-phosphate aldolase -
H681_RS03575 765905..766468 + 564 WP_015475466.1 SEC-C metal-binding protein -
H681_RS03580 766599..767645 - 1047 WP_015475467.1 MBL fold metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9361.91 Da        Isoelectric Point: 10.3172

>T29268 WP_015475462.1 NC_020829:762611-762862 [Pseudomonas sp. ATCC 13867]
MSQHEKLLAKLLNRQAVFTWQELVVLLGGLGYRKLEGDGSRVKFDNGDPCAMINLHKPHPGNELKAYARRQVIEHLKSGG
LIR

Download         Length: 252 bp

>T29268 NC_020829:762611-762862 [Pseudomonas sp. ATCC 13867]
ATGTCTCAGCACGAGAAGCTGCTGGCCAAACTGCTGAACAGGCAGGCTGTGTTCACCTGGCAGGAGTTGGTTGTGCTGCT
GGGTGGGCTGGGTTATCGCAAGCTCGAAGGCGATGGAAGCCGGGTCAAGTTCGACAACGGCGATCCTTGCGCGATGATCA
ATCTGCACAAGCCCCATCCCGGCAACGAATTGAAGGCCTATGCCAGGCGCCAGGTGATCGAGCACCTGAAAAGCGGAGGA
CTGATACGATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12483.09 Da        Isoelectric Point: 4.8329

>AT29268 WP_015475463.1 NC_020829:762859-763200 [Pseudomonas sp. ATCC 13867]
MNNLLQHRGYYGSIEASPEDNCLFGKLLFIRALINYEGDTVAELAGSFRAAVDDYLADCAVRGVEPEIPCKGSFNVRVGH
ELHMAASIAAIQQNITLNDLTRRALSEYIEQHA

Download         Length: 342 bp

>AT29268 NC_020829:762859-763200 [Pseudomonas sp. ATCC 13867]
ATGAACAACCTGCTGCAGCACCGCGGCTATTACGGGTCCATCGAGGCCAGCCCGGAAGACAACTGCCTGTTCGGCAAGCT
GCTGTTCATCCGCGCCCTGATCAATTACGAAGGCGACACCGTCGCCGAGCTGGCCGGCTCGTTCAGGGCAGCGGTCGACG
ACTACCTGGCTGATTGCGCTGTCAGGGGCGTCGAGCCGGAAATCCCCTGCAAGGGCTCCTTCAACGTACGCGTAGGCCAC
GAACTGCACATGGCGGCGAGCATCGCCGCCATCCAGCAGAACATCACGTTGAACGACCTGACGCGCCGGGCGTTGAGTGA
GTACATCGAGCAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4WV53

Antitoxin

Source ID Structure
AlphaFold DB M4WRA3

References