Detailed information of TA system TA029267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 112051..112556
• Replicon: chromosome
• Accession: NC_020829
• Organism: Pseudomonas denitrificans ATCC 13867

Toxin (Protein)

• Gene name: parE
• Uniprot ID: M4WS84
• Locus tag: H681_RS00485
• Protein ID: WP_015474869.1
• Coordinates: 112051..112332 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: M4WTJ9
• Locus tag: H681_RS00490
• Protein ID: WP_015474870.1
• Coordinates: 112329..112556 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H681_RS00465	109042..109992	+	951	WP_015474865.1	alpha/beta hydrolase	-
H681_RS00470	109994..110449	-	456	WP_015474866.1	GNAT family N-acetyltransferase	-
H681_RS00475	110493..111419	-	927	WP_015474867.1	LysR family transcriptional regulator	-
H681_RS00480	111517..111804	+	288	WP_015474868.1	DUF2218 domain-containing protein	-
H681_RS00485	112051..112332	-	282	WP_015474869.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H681_RS00490	112329..112556	-	228	WP_015474870.1	CopG family ribbon-helix-helix protein	Antitoxin
H681_RS00495	112820..114205	+	1386	WP_015474871.1	sodium:solute symporter	-
H681_RS00500	114231..115187	+	957	WP_015474872.1	agmatinase	-
H681_RS00505	115263..116189	+	927	WP_015474873.1	LysR family transcriptional regulator	-
H681_RS00510	116186..116917	+	732	WP_015474874.1	phytanoyl-CoA dioxygenase family protein	-
H681_RS00515	116933..117334	+	402	WP_015474875.1	GFA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10481.93 Da
• Isoelectric Point: 6.4626

>T29267 WP_015474869.1 NC_020829:c112332-112051 [Pseudomonas sp. ATCC 13867]
MSLVWTHKAAADLDAIYDHYVVLIGPEKALRAIQDIVEQVRPLANLDQSSSGVPSEVPGVREWPVERWPYQAAYRVKARS
VQILRIDRVDNSG

Nucleotide

• Length: 282 bp

>T29267 NC_020829:c112332-112051 [Pseudomonas sp. ATCC 13867]
ATGAGCCTGGTGTGGACGCACAAGGCCGCGGCCGACCTGGACGCCATCTACGACCACTACGTGGTGCTGATCGGCCCGGA
GAAGGCGCTGCGGGCCATCCAGGACATCGTCGAGCAGGTTCGTCCGCTGGCGAACCTCGACCAGAGCAGTAGCGGCGTAC
CCAGCGAAGTGCCGGGCGTCAGGGAATGGCCGGTCGAGCGCTGGCCCTACCAGGCCGCCTATCGGGTAAAGGCGCGTTCT
GTACAGATTTTGCGCATCGACCGTGTGGATAACTCGGGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8386.40 Da
• Isoelectric Point: 5.9478

>AT29267 WP_015474870.1 NC_020829:c112556-112329 [Pseudomonas sp. ATCC 13867]
VTTVISFRADDALVQALDQLARATHRDRPYHLRQALAQYLERQSWHVAAIDEGVADADAGHLLEHVVIESKWGLA

Nucleotide

• Length: 228 bp

>AT29267 NC_020829:c112556-112329 [Pseudomonas sp. ATCC 13867]
GTGACCACCGTTATCTCCTTCCGCGCCGATGACGCCCTCGTCCAGGCCCTGGACCAGCTGGCCCGCGCCACCCACCGGGA
CAGGCCCTACCACCTGCGCCAGGCCCTGGCGCAGTACCTGGAGCGCCAGAGCTGGCACGTGGCGGCCATCGATGAAGGCG
TGGCGGACGCCGATGCCGGCCACCTGCTGGAGCACGTCGTCATCGAATCCAAGTGGGGGCTGGCATGA

Structures

Toxin

• AlphaFold DB: M4WS84

Antitoxin

• AlphaFold DB: M4WTJ9