Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 735908..736544 Replicon chromosome
Accession NZ_LS483476
Organism Lederbergia lentus strain NCTC4824

Toxin (Protein)


Gene name mazF Uniprot ID A0A2X4W2Y5
Locus tag DQN84_RS03605 Protein ID WP_066146703.1
Coordinates 736194..736544 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2X4VQT8
Locus tag DQN84_RS03600 Protein ID WP_066146706.1
Coordinates 735908..736189 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQN84_RS03580 732040..732651 - 612 WP_066146717.1 rhomboid family intramembrane serine protease -
DQN84_RS03585 732771..733124 + 354 WP_066146714.1 holo-ACP synthase -
DQN84_RS03590 733387..734403 + 1017 WP_066146713.1 outer membrane lipoprotein carrier protein LolA -
DQN84_RS03595 734561..735724 + 1164 WP_066146709.1 alanine racemase -
DQN84_RS03600 735908..736189 + 282 WP_066146706.1 antitoxin endoai Antitoxin
DQN84_RS03605 736194..736544 + 351 WP_066146703.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DQN84_RS03610 736711..737544 + 834 WP_066146700.1 STAS domain-containing protein -
DQN84_RS03615 737547..737903 + 357 WP_066146760.1 STAS domain-containing protein -
DQN84_RS03620 737907..738308 + 402 WP_066146695.1 anti-sigma regulatory factor -
DQN84_RS03625 738323..739330 + 1008 WP_066146692.1 PP2C family protein-serine/threonine phosphatase -
DQN84_RS03630 739402..739731 + 330 WP_066146690.1 STAS domain-containing protein -
DQN84_RS03635 739731..740204 + 474 WP_066146758.1 anti-sigma B factor RsbW -
DQN84_RS03640 740182..740976 + 795 WP_066146688.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13009.03 Da        Isoelectric Point: 5.7347

>T292612 WP_066146703.1 NZ_LS483476:736194-736544 [Lederbergia lentus]
MAVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDSKRYSFERDSVLLLEQV
RTIDKQRLTDKITHLDEEMMKKVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10648.10 Da        Isoelectric Point: 4.9065

>AT292612 WP_066146706.1 NZ_LS483476:735908-736189 [Lederbergia lentus]
VSESSATTEIIIRLPQQLIAEMDGYAEQENLNHSEIIYRATKVYLRERKKRQIIESMRRGYMEMAKINLAIASEALQAEY
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X4W2Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A2X4VQT8

References