Detailed information of TA system TA292280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2045161..2045690
• Replicon: chromosome
• Accession: NZ_LS483310
• Organism: Staphylococcus aureus strain NCTC9752

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DQL84_RS10625
• Protein ID: WP_000621175.1
• Coordinates: 2045161..2045523 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: DQL84_RS10630
• Protein ID: WP_000948331.1
• Coordinates: 2045520..2045690 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQL84_RS10600	2042139..2042909	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
DQL84_RS10605	2042884..2043363	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
DQL84_RS10610	2043365..2043691	-	327	WP_001052491.1	anti-sigma factor antagonist	-
DQL84_RS10615	2043810..2044811	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
DQL84_RS10625	2045161..2045523	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DQL84_RS10630	2045520..2045690	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DQL84_RS10635	2045775..2046923	-	1149	WP_001281154.1	alanine racemase	-
DQL84_RS10640	2046989..2047348	-	360	WP_000581197.1	holo-ACP synthase	-
DQL84_RS10645	2047352..2047843	-	492	WP_001205912.1	PH domain-containing protein	-
DQL84_RS10650	2047830..2049413	-	1584	WP_001294620.1	PH domain-containing protein	-
DQL84_RS10655	2049406..2049885	-	480	WP_001287079.1	hypothetical protein	-
DQL84_RS10660	2050094..2050654	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T292280 WP_000621175.1 NZ_LS483310:c2045523-2045161 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T292280 NZ_LS483310:c2045523-2045161 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT292280 WP_000948331.1 NZ_LS483310:c2045690-2045520 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT292280 NZ_LS483310:c2045690-2045520 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1