Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4181203..4181700 Replicon chromosome
Accession NZ_LS483303
Organism Escherichia coli strain NCTC9967

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DQM88_RS21460 Protein ID WP_001173343.1
Coordinates 4181203..4181376 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag DQM88_RS21465 Protein ID WP_000398619.1
Coordinates 4181404..4181700 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQM88_RS21430 4176662..4177828 + 1167 WP_111738666.1 hypothetical protein -
DQM88_RS21440 4178675..4180213 + 1539 Protein_3912 lysine decarboxylation/transport transcriptional activator CadC -
DQM88_RS21450 4180411..4181108 - 698 Protein_3913 IS1 family transposase -
DQM88_RS21460 4181203..4181376 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DQM88_RS21465 4181404..4181700 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DQM88_RS21475 4181927..4182199 - 273 WP_000405647.1 N-acetyltransferase -
DQM88_RS21480 4182211..4182441 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
DQM88_RS21485 4182622..4184253 + 1632 WP_001216477.1 sensor histidine kinase -
DQM88_RS21490 4184250..4184969 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4156084..4181700 25616
- flank IS/Tn - - 4180411..4180704 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T292236 WP_001173343.1 NZ_LS483303:c4181376-4181203 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT292236 WP_000398619.1 NZ_LS483303:c4181700-4181404 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References