Detailed information of TA system TA029219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2071202..2071731
• Replicon: chromosome
• Accession: NC_020566
• Organism: Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAI5S5_RS10385
• Protein ID: WP_000621175.1
• Coordinates: 2071202..2071564 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAI5S5_RS10390
• Protein ID: WP_000948331.1
• Coordinates: 2071561..2071731 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAI5S5_RS10360	2068180..2068950	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
SAI5S5_RS10365	2068925..2069404	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAI5S5_RS10370	2069406..2069732	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAI5S5_RS10375	2069851..2070852	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAI5S5_RS10385	2071202..2071564	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAI5S5_RS10390	2071561..2071731	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAI5S5_RS10395	2071816..2072964	-	1149	WP_001281154.1	alanine racemase	-
SAI5S5_RS10400	2073030..2073389	-	360	WP_000581197.1	holo-ACP synthase	-
SAI5S5_RS10405	2073393..2073884	-	492	WP_001205912.1	PH domain-containing protein	-
SAI5S5_RS10410	2073871..2075454	-	1584	WP_001294626.1	PH domain-containing protein	-
SAI5S5_RS10415	2075447..2075926	-	480	WP_001287087.1	hypothetical protein	-
SAI5S5_RS10420	2076134..2076694	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T29219 WP_000621175.1 NC_020566:c2071564-2071202 [Staphylococcus aureus subsp. aureus ST228]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T29219 NC_020566:c2071564-2071202 [Staphylococcus aureus subsp. aureus ST228]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT29219 WP_000948331.1 NC_020566:c2071731-2071561 [Staphylococcus aureus subsp. aureus ST228]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT29219 NC_020566:c2071731-2071561 [Staphylococcus aureus subsp. aureus ST228]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1