Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4237541..4238038 Replicon chromosome
Accession NZ_LS483297
Organism Escherichia coli strain NCTC11023

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DQM98_RS21945 Protein ID WP_001173343.1
Coordinates 4237541..4237714 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag DQM98_RS21950 Protein ID WP_000398619.1
Coordinates 4237742..4238038 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQM98_RS21935 4234211..4235668 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
DQM98_RS21940 4235905..4237422 + 1518 WP_001295074.1 lysine--tRNA ligase -
DQM98_RS21945 4237541..4237714 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DQM98_RS21950 4237742..4238038 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DQM98_RS21960 4238265..4238537 - 273 WP_000405651.1 N-acetyltransferase -
DQM98_RS21965 4238549..4238779 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
DQM98_RS21970 4238960..4240591 + 1632 WP_111732653.1 sensor histidine kinase -
DQM98_RS21975 4240588..4241307 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T292187 WP_001173343.1 NZ_LS483297:c4237714-4237541 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT292187 WP_000398619.1 NZ_LS483297:c4238038-4237742 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References