Detailed information of TA system TA292023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 20263..21173
• Replicon: plasmid 2
• Accession: NZ_LR890752
• Organism: Klebsiella pneumoniae isolate KSB1_8C-sc-2280241

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JMW25_RS25755
• Protein ID: WP_032413375.1
• Coordinates: 20703..21173 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: JMW25_RS25750
• Protein ID: WP_032413443.1
• Coordinates: 20263..20706 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW25_RS25715	16097..17095	+	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-
JMW25_RS25720	17142..18320	+	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
JMW25_RS25725	18457..18804	+	348	WP_032413377.1	hypothetical protein	-
JMW25_RS25730	18862..18987	+	126	Protein_19	transposase	-
JMW25_RS25735	19121..19345	-	225	Protein_20	transposase	-
JMW25_RS25740	19365..19550	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
JMW25_RS25745	19651..20163	+	513	Protein_22	DUF4113 domain-containing protein	-
JMW25_RS25750	20263..20706	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
JMW25_RS25755	20703..21173	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
JMW25_RS25760	21283..21543	-	261	WP_017901409.1	hypothetical protein	-
JMW25_RS25765	22115..22492	-	378	Protein_26	transposase	-
JMW25_RS25770	22626..23594	+	969	WP_162887377.1	IS5 family transposase	-
JMW25_RS25775	23829..25562	-	1734	WP_004118832.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174054	174054
	-	inside	IScluster/Tn	-	-	18832..23594	4762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T292023 WP_032413375.1 NZ_LR890752:20703-21173 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT292023 WP_032413443.1 NZ_LR890752:20263-20706 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4