Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5142358..5142983 Replicon chromosome
Accession NZ_LR890735
Organism Klebsiella pneumoniae isolate INF323-sc-2280125

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMX74_RS25280 Protein ID WP_002882817.1
Coordinates 5142358..5142741 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMX74_RS25285 Protein ID WP_004150355.1
Coordinates 5142741..5142983 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX74_RS25265 5139724..5140626 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMX74_RS25270 5140623..5141258 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMX74_RS25275 5141255..5142184 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMX74_RS25280 5142358..5142741 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMX74_RS25285 5142741..5142983 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMX74_RS25290 5143188..5144105 + 918 WP_004895173.1 alpha/beta hydrolase -
JMX74_RS25295 5144119..5145060 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMX74_RS25300 5145105..5145542 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMX74_RS25305 5145539..5146399 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMX74_RS25310 5146393..5146992 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T292005 WP_002882817.1 NZ_LR890735:c5142741-5142358 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT292005 WP_004150355.1 NZ_LR890735:c5142983-5142741 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References