Detailed information of TA system TA029196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 16775..17396
• Replicon: plasmid pISP4
• Accession: NC_020563
• Organism: Sphingomonas sp. MM-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M4S8P5
• Locus tag: G432_RS21920
• Protein ID: WP_007682056.1
• Coordinates: 17088..17396 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: T0GLH9
• Locus tag: G432_RS21915
• Protein ID: WP_007682049.1
• Coordinates: 16775..17107 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G432_RS23880	12162..12347	-	186	Protein_14	TonB-dependent receptor	-
G432_RS21890	12404..13168	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
G432_RS21895	13229..13696	+	468	Protein_16	aromatic ring-hydroxylating dioxygenase subunit alpha	-
G432_RS21900	13743..14504	+	762	WP_007682039.1	SDR family oxidoreductase	-
G432_RS21905	14550..15218	+	669	WP_081440685.1	EthD domain-containing protein	-
G432_RS23885	15163..15456	-	294	WP_145907151.1	hypothetical protein	-
G432_RS21910	15618..16313	+	696	Protein_20	IS5 family transposase	-
G432_RS21915	16775..17107	-	333	WP_007682049.1	BrnA antitoxin family protein	Antitoxin
G432_RS21920	17088..17396	-	309	WP_007682056.1	BrnT family toxin	Toxin
G432_RS21925	17428..17988	-	561	WP_029987071.1	recombinase family protein	-
G432_RS21930	18033..18797	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
G432_RS21935	18957..19847	-	891	WP_015460628.1	haloalkane dehalogenase	-
G432_RS21940	19933..20187	-	255	WP_007682112.1	hypothetical protein	-
G432_RS21945	20216..20533	+	318	Protein_27	IS6-like element IS6100 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..33183	33183
	-	inside	IScluster/Tn	-	-	6039..20566	14527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-93)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-99)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11771.19 Da
• Isoelectric Point: 6.2230

>T29196 WP_007682056.1 NC_020563:c17396-17088 [Sphingomonas sp. MM-1]
MLSMRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEI
IHIISARKADRKERRRYEEERR

Nucleotide

• Length: 309 bp

>T29196 NC_020563:c17396-17088 [Sphingomonas sp. MM-1]
ATGTTATCCATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGC
CGCCAGCGCCTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGG
GCCTGGTCGAAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATC
ATCCATATCATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12476.26 Da
• Isoelectric Point: 9.8217

>AT29196 WP_007682049.1 NC_020563:c17107-16775 [Sphingomonas sp. MM-1]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Nucleotide

• Length: 333 bp

>AT29196 NC_020563:c17107-16775 [Sphingomonas sp. MM-1]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Structures

Toxin

• AlphaFold DB: A0A126RWR1

Antitoxin

• AlphaFold DB: T0GLH9