Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4504513..4505323 Replicon chromosome
Accession NZ_LR890719
Organism Klebsiella pneumoniae isolate INF161-sc-2280013

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMW82_RS21965 Protein ID WP_004178461.1
Coordinates 4504513..4505046 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW82_RS21970 Protein ID WP_002887278.1
Coordinates 4505057..4505323 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW82_RS21960 4503344..4504465 + 1122 WP_004214138.1 cupin domain-containing protein -
JMW82_RS21965 4504513..4505046 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMW82_RS21970 4505057..4505323 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW82_RS21975 4505426..4506859 - 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW82_RS21980 4506849..4507532 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JMW82_RS21985 4507704..4509089 + 1386 WP_023283179.1 efflux transporter outer membrane subunit -
JMW82_RS21990 4509107..4509451 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T291955 WP_004178461.1 NZ_LR890719:c4505046-4504513 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291955 WP_002887278.1 NZ_LR890719:c4505323-4505057 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References