Detailed information of TA system TA029195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1063683..1064263
• Replicon: chromosome
• Accession: NC_020561
• Organism: Sphingomonas sp. MM-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: M4S2N3
• Locus tag: G432_RS05180
• Protein ID: WP_015457769.1
• Coordinates: 1063967..1064263 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G432_RS05175
• Protein ID: WP_015457768.1
• Coordinates: 1063683..1063964 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G432_RS05130	1058878..1059384	+	507	WP_015457760.1	hypothetical protein	-
G432_RS05135	1059433..1060062	+	630	WP_144061993.1	DNA methyltransferase	-
G432_RS05140	1060062..1060757	+	696	WP_015457762.1	hypothetical protein	-
G432_RS05145	1060787..1061023	+	237	WP_015457763.1	DUF2312 domain-containing protein	-
G432_RS05150	1061026..1061235	+	210	WP_041864799.1	hypothetical protein	-
G432_RS05155	1061232..1061444	+	213	WP_015457764.1	hypothetical protein	-
G432_RS23145	1061441..1062184	+	744	WP_144061994.1	hypothetical protein	-
G432_RS05165	1062147..1062716	+	570	WP_041864800.1	hypothetical protein	-
G432_RS05170	1062764..1063375	+	612	WP_041864801.1	hypothetical protein	-
G432_RS05175	1063683..1063964	-	282	WP_015457768.1	putative addiction module antidote protein	Antitoxin
G432_RS05180	1063967..1064263	-	297	WP_015457769.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G432_RS05185	1064447..1064650	+	204	WP_015457770.1	hypothetical protein	-
G432_RS23910	1064919..1065074	+	156	WP_015457771.1	HNH endonuclease	-
G432_RS05195	1065190..1065609	+	420	WP_015457772.1	hypothetical protein	-
G432_RS05200	1065551..1067446	+	1896	WP_084673621.1	terminase large subunit	-
G432_RS05205	1067443..1068708	+	1266	WP_015457774.1	phage portal protein	-
G432_RS05210	1068665..1069261	+	597	WP_015457775.1	HK97 family phage prohead protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1047136..1095116	47980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11191.96 Da
• Isoelectric Point: 9.9201

>T29195 WP_015457769.1 NC_020561:c1064263-1063967 [Sphingomonas sp. MM-1]
MIEVRRTAEFADWLKGLKDRAAKARIEVRITRLALGNPGQHRNLTKGLREMKIDYATGYRVYFVERGNTVIVLLCGGDKK
SQDRDIARALAMVEDLEE

Nucleotide

• Length: 297 bp

>T29195 NC_020561:c1064263-1063967 [Sphingomonas sp. MM-1]
ATGATCGAAGTCCGCCGCACCGCCGAATTCGCCGACTGGCTCAAGGGCCTCAAGGATCGCGCCGCCAAGGCCCGTATCGA
GGTCCGCATCACCCGGTTGGCGCTGGGCAATCCCGGTCAGCATCGCAACCTGACCAAGGGCCTGCGCGAGATGAAGATCG
ACTACGCCACCGGCTACCGCGTCTATTTCGTGGAGCGGGGCAACACGGTGATCGTCCTGCTTTGCGGCGGGGACAAAAAA
TCGCAGGACCGCGACATCGCCCGCGCGCTGGCGATGGTGGAAGATCTGGAGGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10147.56 Da
• Isoelectric Point: 4.5230

>AT29195 WP_015457768.1 NC_020561:c1063964-1063683 [Sphingomonas sp. MM-1]
MAIKTERWDAAEFLNDEEDIRLYLEAAFEDGEPAIIRAALNDVARARGMTALAKETGLSREALYKALGENGNPTLDTLVK
LTRALGVRLSIAA

Nucleotide

• Length: 282 bp

>AT29195 NC_020561:c1063964-1063683 [Sphingomonas sp. MM-1]
ATGGCTATCAAGACGGAACGGTGGGACGCCGCCGAATTTCTCAACGACGAAGAGGACATCCGCCTCTATCTCGAAGCGGC
ATTCGAGGATGGCGAGCCGGCGATCATCCGCGCCGCGCTGAACGATGTGGCTCGTGCACGCGGCATGACCGCGCTGGCGA
AGGAAACCGGCCTTTCGCGTGAGGCACTCTACAAGGCGCTCGGTGAAAACGGCAACCCGACACTCGACACGCTGGTCAAG
CTCACCCGTGCGCTCGGTGTCCGCCTGTCGATCGCGGCGTGA

Structures

Toxin

• AlphaFold DB: M4S2N3