Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5198656..5199281 Replicon chromosome
Accession NZ_LR890710
Organism Klebsiella pneumoniae isolate KSB2_9B

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMX55_RS25295 Protein ID WP_002882817.1
Coordinates 5198656..5199039 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMX55_RS25300 Protein ID WP_004150355.1
Coordinates 5199039..5199281 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX55_RS25280 5196022..5196924 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
JMX55_RS25285 5196921..5197556 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
JMX55_RS25290 5197553..5198482 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMX55_RS25295 5198656..5199039 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMX55_RS25300 5199039..5199281 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMX55_RS25305 5199486..5200403 + 918 WP_004901674.1 alpha/beta hydrolase -
JMX55_RS25310 5200417..5201358 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMX55_RS25315 5201403..5201840 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMX55_RS25320 5201837..5202697 - 861 WP_002882807.1 virulence factor BrkB family protein -
JMX55_RS25325 5202691..5203290 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T291918 WP_002882817.1 NZ_LR890710:c5199039-5198656 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291918 WP_004150355.1 NZ_LR890710:c5199281-5199039 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References