Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 98305..98830 Replicon plasmid 2
Accession NZ_LR890694
Organism Escherichia coli isolate MSB1_4E-sc-2280348

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW23_RS25145 Protein ID WP_001159868.1
Coordinates 98305..98610 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW23_RS25150 Protein ID WP_000813634.1
Coordinates 98612..98830 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW23_RS25130 94215..95381 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMW23_RS25135 95969..96724 - 756 WP_000852146.1 replication initiation protein RepE -
JMW23_RS25140 97498..98304 - 807 WP_000016982.1 site-specific integrase -
JMW23_RS25145 98305..98610 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW23_RS25150 98612..98830 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW23_RS25155 99538..100533 + 996 WP_000246636.1 hypothetical protein -
JMW23_RS25160 100537..101469 + 933 WP_000991832.1 hypothetical protein -
JMW23_RS25165 101606..102303 - 698 Protein_120 IS1 family transposase -
JMW23_RS25170 102332..103747 - 1416 WP_100498477.1 type I restriction-modification system subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 senB 1..112822 112822
- inside IScluster/Tn - - 101606..110311 8705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T291884 WP_001159868.1 NZ_LR890694:c98610-98305 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT291884 WP_000813634.1 NZ_LR890694:c98830-98612 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References