Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4046609..4047185 Replicon chromosome
Accession NZ_LR890693
Organism Escherichia coli isolate MSB1_4E-sc-2280348

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag JMW23_RS19705 Protein ID WP_001295743.1
Coordinates 4046609..4046896 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag JMW23_RS19710 Protein ID WP_000063148.1
Coordinates 4046883..4047185 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW23_RS19690 4042269..4043573 - 1305 WP_000535012.1 restriction endonuclease subunit S -
JMW23_RS19695 4043563..4045182 - 1620 WP_001029745.1 type I restriction-modification system subunit M -
JMW23_RS19700 4045246..4046412 - 1167 WP_000800831.1 restriction endonuclease -
JMW23_RS19705 4046609..4046896 + 288 WP_001295743.1 BrnT family toxin Toxin
JMW23_RS19710 4046883..4047185 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
JMW23_RS19715 4047219..4048175 - 957 WP_001295745.1 GTPase -
JMW23_RS19720 4048186..4048389 - 204 WP_000467859.1 YbdD/YjiX family protein -
JMW23_RS19725 4048439..4050589 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T291871 WP_001295743.1 NZ_LR890693:4046609-4046896 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT291871 WP_000063148.1 NZ_LR890693:4046883-4047185 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References