Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4455590..4456400 Replicon chromosome
Accession NZ_LR890669
Organism Klebsiella pneumoniae isolate INF321-sc-2280120

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMW44_RS21710 Protein ID WP_004178461.1
Coordinates 4455590..4456123 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW44_RS21715 Protein ID WP_002887278.1
Coordinates 4456134..4456400 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW44_RS21705 4454421..4455542 + 1122 WP_002887282.1 cupin domain-containing protein -
JMW44_RS21710 4455590..4456123 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMW44_RS21715 4456134..4456400 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW44_RS21720 4456503..4457936 - 1434 WP_064179526.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW44_RS21725 4457926..4458609 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMW44_RS21730 4458781..4460166 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
JMW44_RS21735 4460184..4460528 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T291790 WP_004178461.1 NZ_LR890669:c4456123-4455590 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291790 WP_002887278.1 NZ_LR890669:c4456400-4456134 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References