Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1986..2533 Replicon plasmid 3
Accession NZ_LR890653
Organism Escherichia coli isolate MINF_2E-sc-2280463

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag JMW95_RS25050 Protein ID WP_000969996.1
Coordinates 2252..2533 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag JMW95_RS25045 Protein ID WP_000079928.1
Coordinates 1986..2255 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW95_RS25035 46..1077 + 1032 WP_000907875.1 replication initiation protein -
JMW95_RS25040 1773..1931 + 159 WP_000841008.1 hypothetical protein -
JMW95_RS25045 1986..2255 + 270 WP_000079928.1 hypothetical protein Antitoxin
JMW95_RS25050 2252..2533 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JMW95_RS25055 2573..3421 + 849 WP_001057996.1 3'-5' exonuclease -
JMW95_RS25060 3658..3816 - 159 WP_197711125.1 MerR family transcriptional regulator -
JMW95_RS25065 3778..4392 + 615 Protein_6 helix-turn-helix domain-containing protein -
JMW95_RS25070 4401..5261 + 861 WP_000493383.1 EamA family transporter -
JMW95_RS25075 5293..6492 - 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
JMW95_RS25080 6598..7248 + 651 WP_001493764.1 tetracycline resistance transcriptional repressor TetR(A) -
JMW95_RS25085 7280..7522 - 243 WP_000844627.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA2 / cmlA1 / ant(3'')-Ia / sul3 - 1..114568 114568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T291737 WP_000969996.1 NZ_LR890653:2252-2533 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT291737 WP_000079928.1 NZ_LR890653:1986-2255 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References