Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4465641..4466138 Replicon chromosome
Accession NZ_LR890651
Organism Escherichia coli isolate MINF_2E-sc-2280463

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JMW95_RS21735 Protein ID WP_001173343.1
Coordinates 4465641..4465814 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JMW95_RS21740 Protein ID WP_000398619.1
Coordinates 4465842..4466138 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW95_RS21725 4462311..4463768 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
JMW95_RS21730 4464005..4465522 + 1518 WP_001295087.1 lysine--tRNA ligase -
JMW95_RS21735 4465641..4465814 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JMW95_RS21740 4465842..4466138 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JMW95_RS21745 4466365..4466637 - 273 WP_000405649.1 N-acetyltransferase -
JMW95_RS21750 4466649..4466879 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
JMW95_RS21755 4467060..4468691 + 1632 WP_001216487.1 sensor histidine kinase -
JMW95_RS21760 4468688..4469407 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T291721 WP_001173343.1 NZ_LR890651:c4465814-4465641 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT291721 WP_000398619.1 NZ_LR890651:c4466138-4465842 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References