Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5144481..5145106 Replicon chromosome
Accession NZ_LR890643
Organism Klebsiella pneumoniae isolate INF346-sc-2280166

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag JMW14_RS25160 Protein ID WP_004187928.1
Coordinates 5144481..5144864 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMW14_RS25165 Protein ID WP_004150355.1
Coordinates 5144864..5145106 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW14_RS25145 5141847..5142749 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMW14_RS25150 5142746..5143381 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMW14_RS25155 5143378..5144307 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMW14_RS25160 5144481..5144864 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW14_RS25165 5144864..5145106 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMW14_RS25170 5145300..5146217 + 918 WP_004187929.1 alpha/beta hydrolase -
JMW14_RS25175 5146231..5147172 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMW14_RS25180 5147217..5147654 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMW14_RS25185 5147651..5148511 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMW14_RS25190 5148505..5149104 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T291688 WP_004187928.1 NZ_LR890643:c5144864-5144481 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291688 WP_004150355.1 NZ_LR890643:c5145106-5144864 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References