Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 85710..86446 Replicon plasmid 2
Accession NZ_LR890641
Organism Klebsiella pneumoniae isolate INF344-sc-2280162

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMW76_RS25325 Protein ID WP_003026803.1
Coordinates 85710..86192 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMW76_RS25330 Protein ID WP_003026799.1
Coordinates 86180..86446 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW76_RS25305 81555..82988 - 1434 WP_004016055.1 restriction endonuclease -
JMW76_RS25310 83009..83782 - 774 WP_118897055.1 TIGR02391 family protein -
JMW76_RS25320 85018..85329 - 312 Protein_90 hypothetical protein -
JMW76_RS25325 85710..86192 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMW76_RS25330 86180..86446 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMW76_RS25335 86691..87125 - 435 WP_001567367.1 cell envelope integrity protein TolA -
JMW76_RS25340 87172..87720 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
JMW76_RS25345 88214..88522 + 309 WP_017896554.1 hypothetical protein -
JMW76_RS25350 89747..90238 - 492 WP_032249453.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / qnrB1 / dfrA14 / blaCTX-M-15 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B - 1..190324 190324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T291671 WP_003026803.1 NZ_LR890641:c86192-85710 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT291671 WP_003026799.1 NZ_LR890641:c86446-86180 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References