Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3726747..3727372 Replicon chromosome
Accession NZ_LR890640
Organism Klebsiella pneumoniae isolate INF344-sc-2280162

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMW76_RS18060 Protein ID WP_002882817.1
Coordinates 3726747..3727130 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMW76_RS18065 Protein ID WP_004150355.1
Coordinates 3727130..3727372 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW76_RS18045 3724113..3725015 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMW76_RS18050 3725012..3725647 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMW76_RS18055 3725644..3726573 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMW76_RS18060 3726747..3727130 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW76_RS18065 3727130..3727372 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMW76_RS18070 3727577..3728494 + 918 WP_004178029.1 alpha/beta hydrolase -
JMW76_RS18075 3728508..3729449 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMW76_RS18080 3729494..3729931 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMW76_RS18085 3729928..3730788 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMW76_RS18090 3730782..3731381 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T291665 WP_002882817.1 NZ_LR890640:c3727130-3726747 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291665 WP_004150355.1 NZ_LR890640:c3727372-3727130 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References