Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 61657..62393 Replicon plasmid 2
Accession NZ_LR890621
Organism Klebsiella pneumoniae isolate KSB2_1C-sc-2280352

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMX37_RS26175 Protein ID WP_003026803.1
Coordinates 61911..62393 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMX37_RS26170 Protein ID WP_003026799.1
Coordinates 61657..61923 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX37_RS26130 57020..58558 + 1539 WP_201559110.1 IS66 family transposase -
JMX37_RS26135 58848..59111 + 264 WP_009310051.1 hypothetical protein -
JMX37_RS26140 59108..59674 + 567 WP_009310052.1 hypothetical protein -
JMX37_RS26145 59705..60199 + 495 WP_009310053.1 hypothetical protein -
JMX37_RS26150 60243..60611 + 369 WP_009310054.1 hypothetical protein -
JMX37_RS26155 60642..60845 + 204 WP_004098931.1 hemolysin expression modulator Hha -
JMX37_RS26160 60894..61151 + 258 WP_004098928.1 hypothetical protein -
JMX37_RS26165 61227..61481 + 255 WP_004118702.1 hypothetical protein -
JMX37_RS26170 61657..61923 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMX37_RS26175 61911..62393 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMX37_RS26180 62594..63997 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JMX37_RS26185 64026..64658 - 633 WP_001567369.1 hypothetical protein -
JMX37_RS26190 65182..66186 - 1005 WP_000427620.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..230118 230118
- inside IScluster/Tn - - 56223..73507 17284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T291615 WP_003026803.1 NZ_LR890621:61911-62393 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT291615 WP_003026799.1 NZ_LR890621:61657-61923 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References