Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5186965..5187590 Replicon chromosome
Accession NZ_LR890620
Organism Klebsiella pneumoniae isolate KSB2_1C-sc-2280352

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMX37_RS25435 Protein ID WP_002882817.1
Coordinates 5186965..5187348 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMX37_RS25440 Protein ID WP_004150355.1
Coordinates 5187348..5187590 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX37_RS25420 5184331..5185233 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMX37_RS25425 5185230..5185865 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMX37_RS25430 5185862..5186791 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMX37_RS25435 5186965..5187348 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMX37_RS25440 5187348..5187590 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMX37_RS25445 5187795..5188712 + 918 WP_023302328.1 alpha/beta hydrolase -
JMX37_RS25450 5188726..5189667 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMX37_RS25455 5189712..5190149 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMX37_RS25460 5190146..5191006 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMX37_RS25465 5191000..5191599 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T291611 WP_002882817.1 NZ_LR890620:c5187348-5186965 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291611 WP_004150355.1 NZ_LR890620:c5187590-5187348 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References