Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 106829..107354 Replicon plasmid 2
Accession NZ_LR890607
Organism Escherichia coli isolate MINF_8D-sc-2280460

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW69_RS23910 Protein ID WP_001159868.1
Coordinates 106829..107134 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW69_RS23915 Protein ID WP_000813634.1
Coordinates 107136..107354 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW69_RS23895 102793..103959 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMW69_RS23900 104547..105302 - 756 WP_000852146.1 replication initiation protein RepE -
JMW69_RS23905 106022..106828 - 807 WP_000016982.1 site-specific integrase -
JMW69_RS23910 106829..107134 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW69_RS23915 107136..107354 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW69_RS23920 108062..109057 + 996 WP_000246636.1 hypothetical protein -
JMW69_RS23925 109061..109993 + 933 WP_000991832.1 hypothetical protein -
JMW69_RS23930 110130..110827 - 698 Protein_130 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B) senB 1..126618 126618
- flank IS/Tn - - 110130..110633 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T291573 WP_001159868.1 NZ_LR890607:c107134-106829 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT291573 WP_000813634.1 NZ_LR890607:c107354-107136 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References