Detailed information of TA system TA291480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 96986..97725
• Replicon: plasmid 2
• Accession: NZ_LR890584
• Organism: Klebsiella pneumoniae isolate INF145-sc-2279981

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JMW91_RS25950
• Protein ID: WP_065907730.1
• Coordinates: 96986..97471 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: JMW91_RS25955
• Protein ID: WP_012540086.1
• Coordinates: 97459..97725 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW91_RS25920	92435..92965	-	531	WP_065907732.1	antirestriction protein	-
JMW91_RS25925	93590..94423	-	834	WP_040107920.1	type I restriction-modification system subunit M	-
JMW91_RS25930	94470..94700	-	231	WP_038990977.1	hypothetical protein	-
JMW91_RS25935	94788..95135	-	348	WP_038990975.1	hypothetical protein	-
JMW91_RS25940	95202..95582	-	381	WP_038990973.1	hypothetical protein	-
JMW91_RS25945	96486..96644	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
JMW91_RS25950	96986..97471	-	486	WP_065907730.1	GNAT family N-acetyltransferase	Toxin
JMW91_RS25955	97459..97725	-	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
JMW91_RS25960	98025..98348	+	324	WP_065907728.1	metalloregulator ArsR/SmtB family transcription factor	-
JMW91_RS25965	98350..99063	+	714	WP_013087170.1	arsenical resistance protein ArsH	-
JMW91_RS25970	99072..99617	+	546	WP_047365773.1	sigma-70 family RNA polymerase sigma factor	-
JMW91_RS25975	99693..100055	+	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
JMW91_RS25980	100082..101833	+	1752	WP_162875198.1	arsenical pump-driving ATPase	-
JMW91_RS25985	101958..102494	+	537	WP_013087166.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..126908	126908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17840.65 Da
• Isoelectric Point: 9.8560

>T291480 WP_065907730.1 NZ_LR890584:c97471-96986 [Klebsiella pneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKSLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T291480 NZ_LR890584:c97471-96986 [Klebsiella pneumoniae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAAGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT291480 WP_012540086.1 NZ_LR890584:c97725-97459 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT291480 NZ_LR890584:c97725-97459 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AWX8