Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53887..54623 Replicon plasmid 2
Accession NZ_LR890570
Organism Klebsiella pneumoniae isolate INF144-sc-2279979

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMW96_RS25810 Protein ID WP_003026803.1
Coordinates 54141..54623 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMW96_RS25805 Protein ID WP_003026799.1
Coordinates 53887..54153 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW96_RS25760 49949..50311 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JMW96_RS25765 50361..50711 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JMW96_RS25770 51069..51338 + 270 WP_004152102.1 hypothetical protein -
JMW96_RS25775 51326..51901 + 576 WP_004152103.1 hypothetical protein -
JMW96_RS25780 51932..52426 + 495 WP_004152104.1 DNA-binding protein -
JMW96_RS25785 52470..52838 + 369 WP_004152105.1 hypothetical protein -
JMW96_RS25790 52872..53075 + 204 WP_004152106.1 hemolysin expression modulator Hha -
JMW96_RS25795 53124..53381 + 258 WP_004152107.1 hypothetical protein -
JMW96_RS25800 53457..53711 + 255 WP_004152108.1 hypothetical protein -
JMW96_RS25805 53887..54153 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMW96_RS25810 54141..54623 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMW96_RS25815 54782..56036 - 1255 Protein_58 IS3 family transposase -
JMW96_RS25820 56124..57086 - 963 WP_004152113.1 M48 family metalloprotease -
JMW96_RS25825 57073..57561 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
JMW96_RS25830 58059..58256 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186641 186641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T291447 WP_003026803.1 NZ_LR890570:54141-54623 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT291447 WP_003026799.1 NZ_LR890570:53887-54153 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References