Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4578930..4579740 Replicon chromosome
Accession NZ_LR890569
Organism Klebsiella pneumoniae isolate INF144-sc-2279979

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JMW96_RS22355 Protein ID WP_002887280.1
Coordinates 4578930..4579463 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW96_RS22360 Protein ID WP_002887278.1
Coordinates 4579474..4579740 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW96_RS22350 4577761..4578882 + 1122 WP_009309849.1 cupin domain-containing protein -
JMW96_RS22355 4578930..4579463 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
JMW96_RS22360 4579474..4579740 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW96_RS22365 4579843..4581276 - 1434 WP_023287082.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW96_RS22370 4581266..4581949 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JMW96_RS22375 4582122..4583507 + 1386 WP_032414381.1 efflux transporter outer membrane subunit -
JMW96_RS22380 4583525..4583869 + 345 WP_023287080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T291443 WP_002887280.1 NZ_LR890569:c4579463-4578930 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291443 WP_002887278.1 NZ_LR890569:c4579740-4579474 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References