Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 140231..140756 Replicon plasmid 2
Accession NZ_LR890563
Organism Klebsiella pneumoniae isolate INF247-sc-2280148

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JMW77_RS26265 Protein ID WP_001159868.1
Coordinates 140231..140536 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMW77_RS26270 Protein ID WP_000813634.1
Coordinates 140538..140756 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW77_RS26250 136141..137307 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
JMW77_RS26255 137895..138650 - 756 WP_000852146.1 replication initiation protein RepE -
JMW77_RS26260 139424..140230 - 807 WP_000016982.1 site-specific integrase -
JMW77_RS26265 140231..140536 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JMW77_RS26270 140538..140756 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMW77_RS26275 141464..142459 + 996 WP_000246636.1 hypothetical protein -
JMW77_RS26280 142463..143395 + 933 WP_000991832.1 hypothetical protein -
JMW77_RS26285 143532..144229 - 698 Protein_166 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 / aadA5 / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) senB 1..159592 159592
- flank IS/Tn - - 143532..144035 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T291418 WP_001159868.1 NZ_LR890563:c140536-140231 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT291418 WP_000813634.1 NZ_LR890563:c140756-140538 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References