Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4548240..4549050 Replicon chromosome
Accession NZ_LR890562
Organism Klebsiella pneumoniae isolate INF247-sc-2280148

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JMW77_RS22330 Protein ID WP_004178461.1
Coordinates 4548240..4548773 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMW77_RS22335 Protein ID WP_002887278.1
Coordinates 4548784..4549050 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW77_RS22325 4547071..4548192 + 1122 WP_004214138.1 cupin domain-containing protein -
JMW77_RS22330 4548240..4548773 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JMW77_RS22335 4548784..4549050 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMW77_RS22340 4549153..4550586 - 1434 WP_096812520.1 Cu(+)/Ag(+) sensor histidine kinase -
JMW77_RS22345 4550576..4551259 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMW77_RS22350 4551431..4552816 + 1386 WP_047694163.1 efflux transporter outer membrane subunit -
JMW77_RS22355 4552834..4553178 + 345 WP_065519257.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T291410 WP_004178461.1 NZ_LR890562:c4548773-4548240 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291410 WP_002887278.1 NZ_LR890562:c4549050-4548784 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References