Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5290878..5291503 Replicon chromosome
Accession NZ_LR890554
Organism Klebsiella pneumoniae isolate INF182-sc-2280049

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMW27_RS25645 Protein ID WP_002882817.1
Coordinates 5290878..5291261 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMW27_RS25650 Protein ID WP_004150355.1
Coordinates 5291261..5291503 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW27_RS25630 5288244..5289146 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMW27_RS25635 5289143..5289778 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMW27_RS25640 5289775..5290704 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMW27_RS25645 5290878..5291261 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW27_RS25650 5291261..5291503 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMW27_RS25655 5291708..5292625 + 918 WP_012737090.1 alpha/beta hydrolase -
JMW27_RS25660 5292639..5293580 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
JMW27_RS25665 5293625..5294062 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMW27_RS25670 5294059..5294919 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMW27_RS25675 5294913..5295512 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T291383 WP_002882817.1 NZ_LR890554:c5291261-5290878 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT291383 WP_004150355.1 NZ_LR890554:c5291503-5291261 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References