Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4697421..4698231 Replicon chromosome
Accession NZ_LR890542
Organism Klebsiella pneumoniae isolate INF319-sc-2280116

Toxin (Protein)


Gene name KacT Uniprot ID A0A7Z7SK09
Locus tag JMX54_RS23040 Protein ID WP_040190358.1
Coordinates 4697421..4697930 (-) Length 170 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMX54_RS23045 Protein ID WP_002887278.1
Coordinates 4697965..4698231 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX54_RS23035 4696252..4697373 + 1122 WP_012737335.1 cupin domain-containing protein -
JMX54_RS23040 4697421..4697930 - 510 WP_040190358.1 GNAT family N-acetyltransferase Toxin
JMX54_RS23045 4697965..4698231 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMX54_RS23050 4698334..4699767 - 1434 WP_048986922.1 Cu(+)/Ag(+) sensor histidine kinase -
JMX54_RS23055 4699757..4700440 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMX54_RS23060 4700613..4701998 + 1386 WP_048986924.1 efflux transporter outer membrane subunit -
JMX54_RS23065 4702016..4702360 + 345 WP_048986926.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-143)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 170 a.a.        Molecular weight: 18837.54 Da        Isoelectric Point: 6.2333

>T291344 WP_040190358.1 NZ_LR890542:c4697930-4697421 [Klebsiella pneumoniae]
MIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQQKKIPYQN
APSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNLLFYPTKSI
EQLFTDDES

Download         Length: 510 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT291344 WP_002887278.1 NZ_LR890542:c4698231-4697965 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7SK09

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References